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prolong gold antifade reagent containing 4',6-diamidino-2-phenylindole (dapi) dye  (ZSGB Biotech)

 
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    ZSGB Biotech prolong gold antifade reagent containing 4',6-diamidino-2-phenylindole (dapi) dye
    Prolong Gold Antifade Reagent Containing 4',6 Diamidino 2 Phenylindole (Dapi) Dye, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+reagent+containing+dapi+dye/prolong+gold+antifade+reagent+containing+4++6+diamidino+2+phenylindole++dapi++dye/pmc07785802-75-15-19
    Average 90 stars, based on 1 article reviews
    prolong gold antifade reagent containing 4',6-diamidino-2-phenylindole (dapi) dye - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Trichinella spiralis Calreticulin Binds Human Complement C1q As an Immune Evasion Strategy
    Article Snippet: After being washed, the binding of C1q to macrophages was detected using rat anti-C1q mAb (1:100, Abcam) at 4°C overnight and followed by the addition of DyLight 488-labeled goat anti-rat IgG (1:100) (KPL, Milford, MA, USA) for 1 h at 37°C. .. After being stained, the cells adhered to the cell slides were mounted using ProLong Gold Antifade reagent (ZSGB-BIO) containing DAPI dye for the staining of cell nuclei and analyzed by confocal laser scanning microscopy (Leica). .. To determine the effect of r Ts -CRT on the C1q-induced chemotactic migration of macrophages, a transwell containing an insert (Corning, NY, USA) with an 8-μm-pore membrane was used.

    Confocal Laser Scanning Microscopy:

    Article Title: Trichinella spiralis Calreticulin Binds Human Complement C1q As an Immune Evasion Strategy
    Article Snippet: After being washed, the binding of C1q to macrophages was detected using rat anti-C1q mAb (1:100, Abcam) at 4°C overnight and followed by the addition of DyLight 488-labeled goat anti-rat IgG (1:100) (KPL, Milford, MA, USA) for 1 h at 37°C. .. After being stained, the cells adhered to the cell slides were mounted using ProLong Gold Antifade reagent (ZSGB-BIO) containing DAPI dye for the staining of cell nuclei and analyzed by confocal laser scanning microscopy (Leica). .. To determine the effect of r Ts -CRT on the C1q-induced chemotactic migration of macrophages, a transwell containing an insert (Corning, NY, USA) with an 8-μm-pore membrane was used.



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    prolong® gold antifade reagent containing the blue-fluorescent nuclear dye dapi  (Thermo Fisher)
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    Thermo Fisher prolong® gold antifade reagent containing the blue-fluorescent nuclear dye dapi
    Apoptosis/necrosis analysis was performed by an “Apoptosis/Necrosis Detection Kit” (ENZO Life Sciences) on MCF-7 cells that underwent a SCD5 knockdown by a 72-h transient transfection with 60 pmol of SCD5 siRNA oligos. Cells were washed with PBS and incubated with apoptosis (annexin V-EnzoGold)/necrosis (membrane impermeable DNA intercalating dye-7-AAD) dual detection reagent, according to the manufacturer's protocol and observed under an Olympus <t>BX53</t> <t>fluorescent</t> microscope. The concurrent cell staining with annexin V (yellow) and 7-AAD (red) identifies cells in late apoptosis (yellow arrows in the central right panel, orange arrows in the bottom right panel). Staining with the only necrosis red dye (red in the central panels and pink in the bottom merged images, white arrows) detects necrotic cells. Nuclear counterstaining was performed with the blue fluorescent dye <t>DAPI</t> (turquoise arrow). Control siRNA (MCF-7 transfected with non-targeting siRNA oligos). Negative controls (Ct-) were obtained by omitting incubation with dual detection reagent. MCF-7 cells treated with Staurosporine (STS, 2 μM) were used as positive control (Ct+) for necrosis . Images (magnification, 200x) were captured with ISCapture software (Tucsen Photonics) and are representative of three independent experiments. Data are shown as mean + SD of three independent experiments. * p<0.001 vs control siRNA, Student's t test.
    Prolong® Gold Antifade Reagent Containing The Blue Fluorescent Nuclear Dye Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+reagent+containing+dapi+dye/prolong++gold+antifade+reagent+containing+the+blue+fluorescent+nuclear+dye+dapi/pmc05966257-147-30-33
    Average 90 stars, based on 1 article reviews
    prolong® gold antifade reagent containing the blue-fluorescent nuclear dye dapi - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    prolong gold antifade reagent containing 4',6-diamidino-2-phenylindole (dapi) dye  (ZSGB Biotech)
    90
    ZSGB Biotech prolong gold antifade reagent containing 4',6-diamidino-2-phenylindole (dapi) dye
    Apoptosis/necrosis analysis was performed by an “Apoptosis/Necrosis Detection Kit” (ENZO Life Sciences) on MCF-7 cells that underwent a SCD5 knockdown by a 72-h transient transfection with 60 pmol of SCD5 siRNA oligos. Cells were washed with PBS and incubated with apoptosis (annexin V-EnzoGold)/necrosis (membrane impermeable DNA intercalating dye-7-AAD) dual detection reagent, according to the manufacturer's protocol and observed under an Olympus <t>BX53</t> <t>fluorescent</t> microscope. The concurrent cell staining with annexin V (yellow) and 7-AAD (red) identifies cells in late apoptosis (yellow arrows in the central right panel, orange arrows in the bottom right panel). Staining with the only necrosis red dye (red in the central panels and pink in the bottom merged images, white arrows) detects necrotic cells. Nuclear counterstaining was performed with the blue fluorescent dye <t>DAPI</t> (turquoise arrow). Control siRNA (MCF-7 transfected with non-targeting siRNA oligos). Negative controls (Ct-) were obtained by omitting incubation with dual detection reagent. MCF-7 cells treated with Staurosporine (STS, 2 μM) were used as positive control (Ct+) for necrosis . Images (magnification, 200x) were captured with ISCapture software (Tucsen Photonics) and are representative of three independent experiments. Data are shown as mean + SD of three independent experiments. * p<0.001 vs control siRNA, Student's t test.
    Prolong Gold Antifade Reagent Containing 4',6 Diamidino 2 Phenylindole (Dapi) Dye, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+reagent+containing+dapi+dye/prolong+gold+antifade+reagent+containing+4++6+diamidino+2+phenylindole++dapi++dye/pmc07785802-75-15-19
    Average 90 stars, based on 1 article reviews
    prolong gold antifade reagent containing 4',6-diamidino-2-phenylindole (dapi) dye - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    prolong gold antifade reagent containing dapi dye  (ZSGB Biotech)
    90
    ZSGB Biotech prolong gold antifade reagent containing dapi dye
    Apoptosis/necrosis analysis was performed by an “Apoptosis/Necrosis Detection Kit” (ENZO Life Sciences) on MCF-7 cells that underwent a SCD5 knockdown by a 72-h transient transfection with 60 pmol of SCD5 siRNA oligos. Cells were washed with PBS and incubated with apoptosis (annexin V-EnzoGold)/necrosis (membrane impermeable DNA intercalating dye-7-AAD) dual detection reagent, according to the manufacturer's protocol and observed under an Olympus <t>BX53</t> <t>fluorescent</t> microscope. The concurrent cell staining with annexin V (yellow) and 7-AAD (red) identifies cells in late apoptosis (yellow arrows in the central right panel, orange arrows in the bottom right panel). Staining with the only necrosis red dye (red in the central panels and pink in the bottom merged images, white arrows) detects necrotic cells. Nuclear counterstaining was performed with the blue fluorescent dye <t>DAPI</t> (turquoise arrow). Control siRNA (MCF-7 transfected with non-targeting siRNA oligos). Negative controls (Ct-) were obtained by omitting incubation with dual detection reagent. MCF-7 cells treated with Staurosporine (STS, 2 μM) were used as positive control (Ct+) for necrosis . Images (magnification, 200x) were captured with ISCapture software (Tucsen Photonics) and are representative of three independent experiments. Data are shown as mean + SD of three independent experiments. * p<0.001 vs control siRNA, Student's t test.
    Prolong Gold Antifade Reagent Containing Dapi Dye, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+reagent+containing+dapi+dye/prolong+gold+antifade+reagent+containing+dapi+dye/pmc05449505-100-19-17
    Average 90 stars, based on 1 article reviews
    prolong gold antifade reagent containing dapi dye - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    prolong gold antifade reagent contained dapi dye  (Thermo Fisher)
    86
    Thermo Fisher prolong gold antifade reagent contained dapi dye
    Apoptosis/necrosis analysis was performed by an “Apoptosis/Necrosis Detection Kit” (ENZO Life Sciences) on MCF-7 cells that underwent a SCD5 knockdown by a 72-h transient transfection with 60 pmol of SCD5 siRNA oligos. Cells were washed with PBS and incubated with apoptosis (annexin V-EnzoGold)/necrosis (membrane impermeable DNA intercalating dye-7-AAD) dual detection reagent, according to the manufacturer's protocol and observed under an Olympus <t>BX53</t> <t>fluorescent</t> microscope. The concurrent cell staining with annexin V (yellow) and 7-AAD (red) identifies cells in late apoptosis (yellow arrows in the central right panel, orange arrows in the bottom right panel). Staining with the only necrosis red dye (red in the central panels and pink in the bottom merged images, white arrows) detects necrotic cells. Nuclear counterstaining was performed with the blue fluorescent dye <t>DAPI</t> (turquoise arrow). Control siRNA (MCF-7 transfected with non-targeting siRNA oligos). Negative controls (Ct-) were obtained by omitting incubation with dual detection reagent. MCF-7 cells treated with Staurosporine (STS, 2 μM) were used as positive control (Ct+) for necrosis . Images (magnification, 200x) were captured with ISCapture software (Tucsen Photonics) and are representative of three independent experiments. Data are shown as mean + SD of three independent experiments. * p<0.001 vs control siRNA, Student's t test.
    Prolong Gold Antifade Reagent Contained Dapi Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+reagent+containing+dapi+dye/pmc04505495-154-5-12
    Average 86 stars, based on 1 article reviews
    prolong gold antifade reagent contained dapi dye - by Bioz Stars, 2026-10
    86/100 stars
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    Image Search Results


    Apoptosis/necrosis analysis was performed by an “Apoptosis/Necrosis Detection Kit” (ENZO Life Sciences) on MCF-7 cells that underwent a SCD5 knockdown by a 72-h transient transfection with 60 pmol of SCD5 siRNA oligos. Cells were washed with PBS and incubated with apoptosis (annexin V-EnzoGold)/necrosis (membrane impermeable DNA intercalating dye-7-AAD) dual detection reagent, according to the manufacturer's protocol and observed under an Olympus BX53 fluorescent microscope. The concurrent cell staining with annexin V (yellow) and 7-AAD (red) identifies cells in late apoptosis (yellow arrows in the central right panel, orange arrows in the bottom right panel). Staining with the only necrosis red dye (red in the central panels and pink in the bottom merged images, white arrows) detects necrotic cells. Nuclear counterstaining was performed with the blue fluorescent dye DAPI (turquoise arrow). Control siRNA (MCF-7 transfected with non-targeting siRNA oligos). Negative controls (Ct-) were obtained by omitting incubation with dual detection reagent. MCF-7 cells treated with Staurosporine (STS, 2 μM) were used as positive control (Ct+) for necrosis . Images (magnification, 200x) were captured with ISCapture software (Tucsen Photonics) and are representative of three independent experiments. Data are shown as mean + SD of three independent experiments. * p<0.001 vs control siRNA, Student's t test.

    Journal: Oncotarget

    Article Title: Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5

    doi: 10.18632/oncotarget.25273

    Figure Lengend Snippet: Apoptosis/necrosis analysis was performed by an “Apoptosis/Necrosis Detection Kit” (ENZO Life Sciences) on MCF-7 cells that underwent a SCD5 knockdown by a 72-h transient transfection with 60 pmol of SCD5 siRNA oligos. Cells were washed with PBS and incubated with apoptosis (annexin V-EnzoGold)/necrosis (membrane impermeable DNA intercalating dye-7-AAD) dual detection reagent, according to the manufacturer's protocol and observed under an Olympus BX53 fluorescent microscope. The concurrent cell staining with annexin V (yellow) and 7-AAD (red) identifies cells in late apoptosis (yellow arrows in the central right panel, orange arrows in the bottom right panel). Staining with the only necrosis red dye (red in the central panels and pink in the bottom merged images, white arrows) detects necrotic cells. Nuclear counterstaining was performed with the blue fluorescent dye DAPI (turquoise arrow). Control siRNA (MCF-7 transfected with non-targeting siRNA oligos). Negative controls (Ct-) were obtained by omitting incubation with dual detection reagent. MCF-7 cells treated with Staurosporine (STS, 2 μM) were used as positive control (Ct+) for necrosis . Images (magnification, 200x) were captured with ISCapture software (Tucsen Photonics) and are representative of three independent experiments. Data are shown as mean + SD of three independent experiments. * p<0.001 vs control siRNA, Student's t test.

    Article Snippet: After three washes, the appropriate fluorochrome-conjugated secondary antibody was added for 30 min at RT, after which slides were washed and mounted using ProLong® Gold antifade reagent containing the blue-fluorescent nuclear dye DAPI (Thermo Fisher Scientific).

    Techniques: Knockdown, Transfection, Incubation, Membrane, Microscopy, Staining, Control, Positive Control, Software